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. 2019 Jan 31;9:1092. doi: 10.1038/s41598-018-37765-3

Figure 5.

Figure 5

miR-23–27–24 clusters are not essential for endurance-exercise-induced muscle adaptation. (A) Representative images of western blotting analysis. (B) Quantitative data for the protein expression of MyHC type IIa, MyHC type IIb, PGC-1α, and COX IV. Data are means ± SEM (n = 6). **P < 0.01 main effect of exercise. (C) Representative images of SDH staining in plantaris muscle. Scale bar, 100 μm. (D) Representative immunofluorescent images of capillaries in plantaris muscle. Dystrophin is shown in red, CD31 in green. Scale bar, 100 μm. Quantitative data for the capillary-to-fiber ratio (E) and CD31 expression (F) in plantaris muscle. Data are means ± SEM (n = 5). **P < 0.01 main effect of exercise. (G) Representative images of plantaris muscle immunofluorescently stained for MyHC type IIa fibers and dystrophin in green, type IIb fibers in red. MyHC type IId/x fibers are unstained (black). Scale bar, 500 μm. (H) Mean cross-sectional areas (CSAs) of MyHC type IIa, MyHC type Id/x, and MyHC type IIb fibers in plantaris muscle. Data are means ± SEM (n = 4–5). #P < 0.05 main effect of genotype. **P < 0.01 main effect of exercise. (I) Proportions of MyHC IIa, MyHC IId/x, and MyHC IIb fibers in plantaris muscle. Data are means ± SEM (n = 4). #P < 0.05 main effect of genotype. **P < 0.01 main effect of exercise. (J) Expressions of the mature miRNAs in miR-23a/b clusters after 4 weeks of voluntary running. Data are means ± SEM (n = 5). ##P < 0.01 main effect of genotype. *P < 0.05 main effect of exercise. sed, sedentary group; ex, exercised group.