Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 5;16(2):154–164. doi: 10.1038/cmi.2017.160

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

PMC Copyright notice

Increased production of autoantibodies in Siglec-E-deficient mice. (a) Autoantibody staining patterns using indirect fluorescence autoantibody assays in HeLa cells with sera derived from 6-month-old female WT, Siglece ^−/− and B6.NZM ^{Sle1/Sle2/Sle3} (Sle1–3) mice at 1:1000 dilutions (scale bars, 20 μm; original magnification, x60). (b) Sera from 6-month-old female mice showed that ANA levels were markedly increased in Siglece ^−/− mice. WT, N=8; Siglece ^−/−, N=14; Sle1–3, N=7. (c) dsDNA autoantibody levels were assessed by ELISA with sera derived from 6-month-old and 12-month-old female mice. All sera were tested in the same assays. Each point represents a value from an individual mouse, and horizontal bars denote means with standard error of the mean (s.e.m.). Sample sizes were as follows: 6 months: WT, N=8; Siglece ^−/−, N=14; Sle1–3, N=7. 12 months: WT, N=6; Siglece ^−/−, N=12; Sle1–3, N=5. P-values at 6 months were analyzed using Mann–Whitney tests and were found to be nonsignificant. P-values at 12 months were calculated with a two-tailed unpaired Student’s t-test. Errors bar show deviations in biological repeats. ANA, antinuclear antibody; dsDNA, double-stranded DNA; ELISA, enzyme-linked immunosorbent assay; Siglec, sialic acid-binding immunoglobulin-like lectin; WT, wild type.