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. 2019 Jan 31;9:1044. doi: 10.1038/s41598-018-38026-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Two-dimensional electrophoresis of total proteins from bacteria. Total proteins were extracted from untreated and plasma treated (air plasma for 3 min) bacteria, and then applied to two-dimensional electrophoresis. The upper and bottom gels show 2-dimentional profiles of total proteins from untreated and plasma treated bacteria, respectively. Seven protein spots in which the level was increased in plasma treated bacteria more than 3-fold are indicated in green circles (numbered as 1–7) on both gels. Seven protein spots in which the level was decreased in plasma treated bacteria more than 3-fold are indicated in red circles (numbered as 8–14) on both gels. These 14 protein spots were used for protein identification.