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. 2019 Jan 31;9:50. doi: 10.1038/s41398-018-0335-z

Fig. 2. Protein levels in PBMCs correlate with the severity of the clinical phenotype.

Fig. 2

Bar plots representing the quantification of the technical replicates in the three subgroups, CTRL, Mild ASD and Severe ASD show significantly increased levels of a p-eIF4E (F(2,45) = 9.51; p = 0.0004) b rpS6 (F(2,38) = 3.70; p = 0.03) and c p-MNK1 (F(2,29) = 5.02; p = 0.01) in the severe subgroup compared to controls, while d TSC1 levels were significantly increased in the mild group only (F(2,45) = 3.24; p = 0.048). Error bars represent the standard error of the mean (n = 11-21 CTRL, 6-7 Mild ASD, 11-20 Severe ASD; *p < 0.05, **p < 0.01, ***p < 0.001; one-way ANOVA). e Scatter plot of the two canonical functions containing four proteins (p-eIF4E, rpS6, TSC1, p-MNK1) that discriminated subjects according to their severity into three groups: CTRL, mild ASD and severe ASD (Wilks’ Lambda = 0.480; Chi-square = 20.2; df = 8; p = 0.01). The mean discriminant scores for each group are depicted as group centroids. Four patients were not included in this analysis (see Table 1)