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. 2019 Jan 31;9:1072. doi: 10.1038/s41598-018-37788-w

Figure 8.

Figure 8

Cell-cell and cell-matrix interactions guide endothelial morphogenesis in colloidal gels. (a) Levels of focal adhesion kinase (FAK), phosphorylated FAK (p-FAK), and VE-cadherin in endothelial cells measured by immunoblot analysis. β-actin as loading control in N-, P- and AB-gel (a) with 0.05 particle fraction, and (c) with 0.2 particle fraction, at 48 hrs. Quantified level FAK, p-FAK and VE-cadherin normalized to β-actin shows differential level of markers in endothelial cells in N-, P- and AB-gel (b) with 0.05 particle fraction, and (d) with 0.2 particle fraction, at 48 hrs (**P ≤ 0.01, *P ≤ 0.05, ANOVA). (e) Relative change of FAK, and VE-cadherin in endothelial cells in N-, P- and AB-gel with 0.2 particle fraction with respect to gels with 0.05 particle fraction. The corresponding full length blots are represented in Supplementary Fig. S8.