Fig. 1.

Rix7 is an essential type II AAA-ATPase. a Cartoon schematic of the three domains of S. cerevisiae (SC) Rix7 including the N-terminal domain (NTD, gray), the D1 AAA domain (light blue), and the D2 domain (light orange). Shown in the inset is the secondary structure composition of the D1 AAA domain. Each AAA domain contains an α/β subdomain including the Walker A (red), Walker B (yellow), sensor 1 (blue) and arginine finger (purple) motifs and an all α-helical lid subdomain (α5-α8). The pore loop 1 and pore loop 2 are indicated as PL-I and PL-II, respectively. This figure was adapted from Hanzelmann and Schindelin, 2016²⁵. b Growth curves of S. cerevisiae tetO₇-RIX7 strains transformed with plasmids encoding wild type Rix7, mutants of Rix7, and the ARS1-CEN4 YCplac vector with no insert. Strains were grown in the absence (black) or presence (green) of doxycycline at 25 °C and the absorbance was recorded at 595 nm over a 25 h time period. Each curve is the average of three independent replicates and the error bars mark the standard deviation between replicates. Source data are provided as a source data file