Fig. 2.

ATP hydrolysis by Rix7 is required for ribosome assembly in S. cerevisiae. Sucrose gradient polysome profiles of select tetO7-RIX7 strains grown in the presence of doxycycline. Position of peaks for the 40S subunit, 60S subunit, 80S monosome, and polysomes are indicated on the top profile as a cartoon. The red arrow indicates a decrease in 60S subunits and the dashed lines indicate the presence of halfmers (engaged 40S subunits that lack a 60S subunit as shown in cartoon)