Fig. 3.

Rix7 forms an asymmetric closed ring double hexamer. a Top, side, and bottom views of the final Rix7 cryo-EM reconstruction shown in gray. The pseudo atomic model of Rix7 is shown overlaid with the reconstruction. Individual AAA protomers are colored as follows P1 (green), P2 (cyan), P3 (orange), P4 (purple), P5 (yellow), and P6/Seam (red). b View of the individual protomers from the hexamer. The view from each protomer was generated by successive 60° rotations along the y axis. Dashed lines are defined as the highest position of D1 domain (P1 protomer), the linker position between D1 and D2 (P1 protomer), and the lowest position of D2 domain (P5 protomer). c The pseudo atomic model of the Rix7 P1 protomer shown as a cartoon with the cryo-EM reconstruction overlaid. This illustrates the fit of the individual protomer within the cryo-EM map and the quality of the reconstruction. Also shown is the additional density observed for the polypeptide in the pore. The pore loop 1 and pore loop 2 are indicated as PL-I and PL-II, respectively in both the D1 and D2 domains. The position of ATP in the nucleotide binding pocket is indicated