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. 2019 Feb 1;6:20. doi: 10.1038/s41438-018-0107-1

Fig. 1. Vector construct for genome editing of the BoPDS, BoSRK3 and BoMS1 genes.

Fig. 1

a, c, e Diagram of the cabbage BoPDS, BoSRK3, and BoMS1 genes, respectively, with the four target sites indicated. b, d Diagram illustrating the engineered CRISPR/Cas9 vector with the tRNA-processing system based on multiplex sgRNAs for the BoPDS and BoSRK3 genes. f Diagram illustrating the tRNA-sgRNA intermediate vector for the BoMS1 gene. g Diagram illustrating the engineered CRISPR/Cas9 vector for the BoMS1 and BoSRK3 gene editing. The blue boxes indicate exons, and the black lines indicate introns. The target sequence is shown in black letters. PAM is marked in red letters. The primer sites used to evaluate the mutation types are shown by black arrows. The screening marker gene expression cassette of Bar was not displayed in the T-DNA region (b, d, g)