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. 2019 Feb 1;6:20. doi: 10.1038/s41438-018-0107-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a, b Comparison of the lower, stamen, pistil, anther, and pollen development between the wild type and the C-4 mutant. a1–a3 floral organ morphology of the wild type; b1–b3 floral organ morphology of the C-4 mutant; a4 pollen formation in the anther of the wild type (10 × 20); b4 empty pollen sac in the anther from the C-4 mutant (10 × 20). c cross-compatibility between the C-4 mutant and the wild type with the same S-allele. c1 in situ fluorescence microscopy of pollen (from the wild type) germination on the stigma of the C-4 mutant at the bud stage; c2 in situ fluorescence microscopy of pollen (from the wild type) germination on the stigma of the C-4 mutant at the flowering stage; c3–c5 silique growth and seed-set of the C-4 mutant × wild type at the bud stage; c6-c8 silique growth and seed set of the C-4 mutant plant × wild type at the flowering stage. d Self- or cross-compatibility indices of the BoMS1 and BoSRK3 gene mutant plants. Bars represent the mean ± standard deviation (SD)