Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Dec 4;29(2):136–150. doi: 10.1038/s41422-018-0120-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — Ubiquitinated Rheb promotes its interaction with TSC. a Co-IP assay was performed to analyze the binding of TSC2 to different forms of Rheb mutants (active form: S16H; inactive form: D60V, D60K, S20N). b TSC2 purified from HEK293T cells showed stronger binding affinity to the ubiquitinated Rheb. c Rheb-K8R mutant showed weaker binding affinity to TSC2 as compared with Rheb-WT. d, e RNF152 Knockdown (d) or knockout (e) reduced the binding bewteen Rheb and TSC2. The knockdown efficiency was detected by RT-PCR in Supplementary information, Fig. S2e. RNF152-KO MEF cells were analyzed via genomic DNA PCR in Supplementary information, Fig. S3s. f–h USP4 knockdown (f), knockout (g) or inactivation of USP4 by Vialinin A (h) promoted the binding of Rheb to TSC2