Fig. 7.

CDK9 inhibition reduced osteosarcoma cell clonogenicity and migration in vitro and decreased the spheroid diameter of osteosarcoma cell lines in a 3D cell culture. (A) Representative results of colony formation in U2OS and KHOS. The numbers of colonies and their sizes were markedly decreased in cells treated with LDC067. Representative images of (B) U2OS, and (C) KHOS cell migration after LDC067 treatment for 24 h, 48 h, and 72 h. Cell migration distance of osteosarcoma cell lines (D) U2OS, and (E) KHOS were measured after 10 μM of LDC067 treatment. Spheroid formation of (F) U2OS, and (G) KHOS were significantly smaller than untreated cells at all observation points. Cells fluorescence images of spheroid formation were taken after 12 days of cultivation. The curves of relative spheroid diameter of (H) U2OS, and (I) KHOS treated with CDK9 inhibitor compared with untreated cells at all observation points. The data are mean ± SE of the 2 experiments carried out in triplicate (Scale bar, 100 μm). (** indicates P < .01).