Fig. S3.

CDK9 inhibition by siRNA transfection decreases osteosarcoma cell proliferation and migration. CDK9 expression and osteosarcoma cell proliferation of (A) U2OS and (B) KHOS were determined by immunofluorescence after an incubation with a CDK9 antibody (green) and β-Actin (red). Representative images of (C) U2OS, and (D) KHOS cell migration after CDK9 siRNA and negative control (NC) siRNA transfection for 0 h, 24 h, 48 h, and 72 h. The distance of cell migration of the osteosarcoma cell lines (E) U2OS, and (F) KHOS were measured after siRNA transfection. (Scale bar, 50 μm).