Fig. S4.

CDK9 inhibition induced osteosarcoma cell apoptosis measured by flow cytometry analysis. Representative images of cell apoptosis in osteosarcoma cell lines (A) U2OS and (B) KHOS treated with the 10 μM of CDK9 inhibitor. (C and D) Representative bar chart showed apoptosis rate (reflect by the Annexin VP-E positive cells) of cell lines U2OS and KHOS, respectively, after CDK9 inhibitor treatment compared with untreated cells. The data are mean ± SE of the 2 experiments carried out in triplicate (* indicates P < .05).