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. 2019 Jan 31;9:1032. doi: 10.1038/s41598-018-37648-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ERα promotes and ERβ inhibits the proliferation, invasion and migration of human PTC cells and normal thyroid cells. BCPAP, K1 and Nthy-ori3-1 cells were stably transfected with the expression vectors of ERα-shRNA, ERβ-shRNA and scrambled shRNA or were exposed to 10 nM of E2, PPT and DPN for 72 h, respectively. (A) Knockdown of ERα and ERβ protein levels by ERα-shRNA and ERβ-shRNA. (B and C) The proliferation of these cells was assessed by BrdU incorporation and cell count assays. (D and F) The migration and invasion of these cells were evaluated by Transwell assay. Data presented represent the mean of three independent experiments. Statistical differences between two groups were examined using Students t-test. *P < 0.05, compared with non treatment (Veh).