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. 2019 Jan 31;9:1032. doi: 10.1038/s41598-018-37648-7

Figure 4.

Figure 4

Effects of PES1 on the ERα/ERβ protein ratio and the proliferation, invasion and migration of human PTC and normal thyroid cells. The normal thyroid-derived Nthy-ori3-1 cells were stably transfected with the PES1 expression vector or empty vector and the PTC-derived BCPAP and K1 cells were stably transfected with the expression vector of PES1-shRNA or scrambed shRNA. The protein levels of PES1, ERα and ERβ in these stable transfected cells were assessed by Western blotting. β-actin served as an internal calibrator. The proliferation, invasion and migration of these stable transfected cells were assayed after exposure to E2 for 72 h. (A) Blot examples of PES1, ERα and ERβ protein levels in these stable transfected cells. (B) Bar diagrams of relative PES1 protein level in these stable transfected cells. (C) The concentrations of ERα and ERβ protein in these stable transfected cells. (D and E) The proliferation of these stable transfected cells was assessed by BrdU incorporation and cell count assays. (F and G) The migration and invasion of these stable transfected cells were assessed by Transwell assay. Data presented represent the mean of three independent experiments. Statistical differences between two groups were examined using Students t-test. *P < 0.05, compared with non treatment (Veh). #P < 0.05, compared with E2 treatment alone.