Identification and validation of the altered expression of RNA-binding protein genes in cervical cancer. (A) Heat map of the 95 differentially expressed RBP genes with normalized expression values across 7 normal cervical tissue samples and 7 cervical cancer tissue samples. (B) RNA-seq expression values (in RPKM) for selected 8 RBP genes. (C) TaqMan RT-qPCR validation of 8 RBP genes in 24 normal cervix samples, 25 cervical precursor lesion samples (CIN2 to CIN3), and 23 cervical cancer tissue samples. Data are shown as means (2−ΔΔCt) ± SD. NS, not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001. (D and E) ROC curves of 8 RBP gene expression detection, showing good discrimination of cancer group from CIN2-CIN3 plus normal group (D) and cancer plus CIN2-CIN3 group from normal group (E).