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Experimental conditions for measuring BPAF glucuronidation kinetics.
| Enzyme | Substrate concentrations (µM) | Enzyme concentration (mg/mL) | Incubation times (min) |
|---|---|---|---|
| UGT1A3 | 1, 2.5, 5, 10, 20, 30, 40, 50 | 0.01 | 20 |
| UGT1A10 | 0.25, 1, 2.5, 5, 10, 15, 20, 30, 40, 50 | 0.01 | 10 |
| UGT2A1 | 1, 2.5, 5, 10, 20, 30, 40, 50 | 0.01 | 10 |
| UGT2B7 | 0.25, 1, 1.5, 2.5, 5, 10, 20, 30, 40 | 0.05 | 30 |
| UGT2B17 | 0.1, 0.5, 1, 2.5, 5, 10, 20, 30, 40, 50 | 0.01 | 10 |
