Figure 5.

CDX1 Overexpression Attenuated EMT in Trophoblasts

(A) Overexpression of CDX1 increased the expression of E-cadherin and reduced the N-cadherin level by western blot analysis. (B) Statistical analysis of the western blot results in (A). (C and D) CDX1 overexpression significantly decreased trophoblast cell invasion. After transfection for 48 h, the overexpression of CDX1 attenuated the invasion of trophoblast cells in comparison with that of the control group. CDX1 overexpression also enhanced EZH2 siRNA-mediated deficient cell invasion. (D) is the statistical result of (C). The scale bar represents 200 μm. All data are reported as the mean ± SEM. *p < 0.05; **p < 0.01. EMT, epithelial-mesenchymal transition.