Fig. 5.

Localization of TEX101 and LY6K proteins in human spermatozoa. Immunofluorescence images were obtained with a 100× oil immersion objective. TEX101 (A) or LY6K (G) were labeled with primary antibodies and visualized with secondary antibodies conjugated to Alexa Fluor 594 (red). No primary antibodies were used for the negative controls (D and J). Spermatozoa nucleus (blue) was visualized with DAPI (B, E, H, and K). Panels C, F, I, and L represent merged brightfield and Alexa Fluor 594 fluorescence images. TEX101 and LY6K were localized in the neck, postequatorial and equatorial regions of spermatozoa. In some spermatozoa, LY6K was also localized in the acrosomal region. In the equatorial and postequatorial regions TEX101 and LY6K were localized to the plasma membrane.