Fig. 5.

Validation of selected lysine-succinylated proteins in A. hydrophila using Co-Immunoprecipitation and Western blotting. Total proteins of A. hydrophila were used for Western blotting with anti-specific target antibodies as lane inputs (left panel). Candidate proteins were enriched by Co-IP with specific antibodies and followed by Western blotting with specific antibodies (middle panel). Candidate proteins were enriched by Co-IP with specific antibodies and followed by Western blotting with anti-lysine succinylation antibodies (right panel). Pre-immune serum of rabbit antibody was used as the negative controls in both experimental process (left side of each lane in middle and right panel).