Fig. 7.

H. pylori up-regulates RABEP2 and G3BP2 in human gastric epithelial cells in vitro. AGS human gastric epithelial cells were cocultured with wild-type cag+ H. pylori strain 7.13, a 7.13 cagE-isogenic mutant, or a 7.13 cagA-isogenic mutant at a multiplicity of infection (MOI) of 100:1 for 24 h. Western blot analysis was used to assess RABEP2 (A) or G3BP2 (B) protein expression relative to GAPDH protein expression. RABEP2 and G3BP2 protein expression levels were standardized to GAPDH expression, and densitometry was used to quantify all independent replicates. Data are represented as fold over uninfected (UI) control. Error bars indicate standard error of the mean from experiments performed on at least three independent occasions, and ANOVAs were used to determine statistical significance among groups. *, p < 0.05; **, p < 0.005; ***, p < 0.0005; ****, p < 0.0001.