Table 1.
Overview of Samorin fractions and control drugs against T. congolense and T. b. brucei.
| compound |
T. congolense IL3000 |
T. brucei s427 |
T. b. brucei B48 |
T. b. brucei ISMR1 |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EC50 (μM) | n | EC50 (μM) | n | EC50 (μM) | n | RF (s427) | P value (s427) | EC50 (μM) | n | RF (s427) | P value (s427) | |
| 1A | 0.035 ± 0.005 | 5 | 3.48 ± 0.42 | 4 | 1.85 ± 0.19 | 4 | 0.53 | 0.012 | 5.64 ± 0.40 | 4 | 1.62 | 0.0093 |
| 1B | 0.65 ± 0.06 | 5 | 5.31 ± 0.48 | 4 | 5.58 ± 0.07 | 4 | 1.05 | 0.59 | 7.14 ± 0.14 | 4 | 1.34 | 0.010 |
| 2 | 0.035 ± 0.005 | 5 | 2.67 ± 0.48 | 4 | 1.06 ± 0.11 | 4 | 0.40 | 0.017 | 4.97 ± 0.22 | 4 | 1.86 | 0.0047 |
| 3 | 0.36 ± 0.02 | 5 | 12.2 ± 1.4 | 6 | 7.13 ± 0.43 | 6 | 0.59 | 0.005 | 17.4 ± 1.4 | 6 | 1.43 | 0.025 |
| 4 | 16.0 ± 0.7 | 6 | 95.7 ± 17.0 | 4 | 86.1 ± 3.3 | 4 | 0.90 | 0.60 | 96.4 ± 24.6 | 4 | 1.01 | 0.98 |
| Samorin | 0.0033 ± 0.0006 | 17 | 0.29 ± 0.04 | 12 | 0.020 ± 0.005 | 10 | 0.068 | 0.00001 | 1.55 ± 0.05 | 14 | 5.33 | >0.00001 |
| Ethidium | 0.11 ± 0.01 | 5 | 3.85 ± 0.22 | 4 | 5.03 ± 0.17 | 4 | 1.31 | 0.0051 | 7.41 0.23 | 4 | 1.92 | 0.00003 |
| Pentamidine | 0.82 ± 0.02 | 11 | 0.0051 ± 0.0004 | 6 | 0.63 ± 0.03 | 6 | 124 | >0.00001 | 0.016 ± 0.002 | 6 | 3.24 | >0.00001 |
The 50% Effective Concentration (EC50) is given as average and SEM, and the number of replicates (n) for each determination is included in a separate column. RF is the Resistance Factor, the ratio of EC50 (s427) and EC50 (B48 or ISMR1). The P value is the outcome of an unpaired, two-tailed Student's t-test of the B48 or ISMR1 EC50 with the corresponding value of s427.