Figure 3.

Silencing of snail reverses HGF‐induced sorafenib resistance. (A) SMMC‐7721 and HepG2 cells transfected with CTL‐siRNA or snail‐siRNA were incubated with HGF (10 ng·mL⁻¹) and protein levels of Snail (3 h after incubation), E‐cadherin and vimentin (48 h after incubation) were detected. The density of each band was normalized to GAPDH (*P < 0.05, compared to HGF). (B and C) SMMC‐7721 and HepG2 cells transfected with CTL‐siRNA or snail‐siRNA were incubated with sorafenib with or without HGF pre‐treatment (10 ng·mL⁻¹) and cell viability was detected by the CCK‐8 assay (*P < 0.05, **P < 0.01, CTL‐siRNA+HGF vs. snail‐siRNA+HGF). Data are expressed as the mean ± SD from three individual experiments. Differences between groups were determined using Student's t‐test and two‐way ANOVA with Bonferroni correction.