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. 2018 Dec 29;11(1):19. doi: 10.3390/v11010019

Figure 3.

Figure 3

Clonality and mutational characteristics of the ZIKV plasmablast response. (A) Clonal relatedness of heavy chain variable region (VH) sequences amplified from patient plasmablasts. The number at the center of each pie chart is the total number of heavy chain sequences analyzed, including unpaired VH sequences that were not pursued for mAb synthesis. The numbers within the pie slices denote the number of clones with a shared VH rearrangement and junction. The percentage of total clonal sequences per donor is shown to the bottom right of the pie charts. (B) Average per donor VH mutation frequencies of the two ZIKV patients compared to historical data. Each circle represents the average number of VH nucleotide mutations per donor. Somatic hypermutation frequencies in naive, memory and germinal center B cells, and peripheral B cells from influenza and dengue infected donors were derived from previously published data [33,42]. (C) The range of VH mutation frequencies in the two ZIKV patients. Each circle represents the number of VH mutations per mAb sequence. The binding specificities of the mAbs to ZIKV and DENV are color coded: Cross-reactive mAbs are shown in blue, ZIKV-reactive in green and mAbs of undetermined specificity in orange. (D) VH CDR3 amino acid length distribution of all mAb sequences per patient.