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. 2019 Jan 11;10(1):39. doi: 10.3390/genes10010039

Figure 1.

Figure 1

Co-delivery of TALENs and donor DNA using helper-dependent adenoviral (HD-Ad) vectors. (A) Schematic representation of HD-Ad vector-mediated integration of a gene cassette for expressing the LacZ reporter or human cystic fibrosis transmembrane conductance regulator (CFTR) into Exon 2 of the adeno-associated virus integration site 1 (AAVS1) locus. (B) Transduction of IB3-1 cells with a negative control (HD-Ad-K18LacZ) vector at 100 MOI and HD-Ad-UBCLacZ-TALEN vector at 50 and 100 MOI. X-gal staining detects cells with LacZ expression. (C) T7E1 assay for assessing the cleavage efficiency of cells transduced with the HD-Ad-K18LacZ-TALEN vector. Human IB3-1 cells were transduced with the vector at 50 MOI (Lane 2, 5) and 100 MOI (Lane 3, 6). Cells transduced with 100 MOI of HD-Ad-K18LacZ (Lane 1, 4) were used as negative controls. Lanes 1–3 were samples with T7 endonuclease treatment, while Lanes 4–6 were samples with no T7 endonuclease treatment. The arrow indicates the uncut PCR product.