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Disappearance of TALEN expression and vector genome over time. (A) GFP fluorescence at different time points following HD-Ad-K18CFTR-TALEN vector transduction 100 MOI. (B) qPCR quantification of TALEN mRNA levels, as well as vector genome in IB3-1 cells transduced with HD-Ad-K18CFTR-TALEN vector. N = 3. (C) Western blot analysis of TALEN protein expression at different time points post transduction using the JESS system.
