MRM chromatograms obtained by injecting a mixture containing 60 pg each cycloartenyl ferulate (m/z 601.425 [M − H]−), 24-methylenecycloartanyl ferulate (m/z 615.441 [M − H]−), campesteryl ferulate (m/z 575.410 [M − H]−), and β-sitosteryl ferulate (m/z 589.425 [M − H]−) standards (A). Representative MRM chromatograms of cycloartenyl ferulate, 24-methylenecycloartanyl ferulate isomers, campesteryl ferulate, and β-sitosteryl ferulate in mouse plasma (B) or liver (C) of the HOZ-HF group. A 5 μL plasma or liver extract was injected into the HPLC-MS/MS. Detailed analytical conditions are described in the Materials and methods section.