Figure 2.

The effect of DPHC treatment on adipogenic-specific protein levels in 3T3-L1 adipocytes. The 3T3-L1 preadipocytes were incubated in a differentiation medium with or without the indicated concentrations of DPHC for eight days (from day 0 to day 8). The expression of sterol regulatory element-binding protein 1c (SREBP-1c), peroxisome proliferator-activated receptor-γ (PPARγ), CCAAT/enhancer-binding protein α (C/EBPα), and adiponectin were assessed by Western blotting (A). The expression of perilipin, fatty acid synthase (FAS), fatty acid binding protein (FABP4), and leptin were assessed by Western blotting (B). Immunoblot figures are representative of three independent experiments, and each value is expressed as the mean ± SE of three determinations. Significant differences from the control group were identified at * p < 0.05.