Figure 1.
SAHA and EGCG diminish cell density when seeded on a 6-well plate. Cells (2 × 105) were plated on a 6-well plate and treated with SAHA and EGCG alone or in combination (3 μM SAHA + 5 μM EGCG) for 72 h. SAHA and EGCG administered singly were able to reduce the cell density, but not as much as the combination of 3 μM SAHA and 5 μM EGCG. With this combination treatment, not only were there fewer cells present on the plate, but the cells that were visible appeared to be more fibroblastic, suggesting a more highly differentiated state. There was no apparent change in cell density with the treatments in the MCF10A cells. All photos were taken at 100× magnification.