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. 2018 Dec 27;11(1):23. doi: 10.3390/cancers11010023

Figure 6.

Figure 6

Acetylated histone H3 is enriched in the promoter region of p27 with the combination treatment of SAHA and EGCG. (A) ChIP qRT-PCR analysis using AcH3 antibodies (Millipore Sigma, Burlington, MA, USA) and p27 promoter primers (Integrated DNA Technologies, Coralville, IA, USA) was performed with a ChIP kit (Abcam, Cambridge, MA, USA) in MCF-7 cells after 3-day treatments with the indicated compounds (n = 3). (B–D) The same was done in the MDA-MB-157, MDA-MB-231, and HCC1806 triple-negative cell lines. Histone H3 antibody (Abcam) was used as a positive control for comparison.