An n-6HFD (diet high in n-6 fatty acids) increases body weight and increases the expression of farnesoid-X-receptor (Fxr) in mouse colonic mucosa. (A) Weaned C57/BL6 male mice (n = 6) were assigned to a control diet containing 13% energy (13%E, 5% by weight safflower oil (SO), 76% linoleic acid (LA)) or an n-6HFD containing 44 energy (44%E, 22% by weight SO) for 6 weeks. Bars represent means ± standard error of the mean (SEM) of quantitation (fold change of control) of (B) Fxr and peroxisome proliferator-activated receptorγ1 (Pparγ1), (C) ileal bile acid-binding protein (Ibabp), and (D) small heterodimer protein (Shp) mRNA corrected for glyceraldehyde dehydrogenase phosphate (Gapdh) mRNA as an internal standard. Means ± SEM (standard error of the mean) with an asterisk (*) differ (p < 0.05).