Figure 2.

An n-6HFD induces Fxr CpG demethylation in mouse colonic mucosa. (A) Organization of the mouse Fxr gene. The top arrows indicate transcription start sites (+1) on exon-1 and exon-3. The bottom arrows indicate positions of oligonucleotides (−54/+416) around exon-3 used for CpG methylation studies [25]. (B) PCR bands amplified from bisulfonated genomic DNA obtained from proximal colonic mucosa with mouse Fxr- and β-actin-methylation (M)-specific primers. MC = methylation control. (C) Quantitation (fold-change/control) of Fxr promoter methylation status with control (n = 5) and n-6HFD (n = 6). Means ± SEM with an asterisk differ (p < 0.05).