Figure 5.

An n-6HFD induces Apc CpG hypermethylation in mouse colonic mucosa. (A) Quantitation (fold-change/control) of Apc promoter methylation status with control (n = 5) and n-6HFD (n = 6). (B) and (C) The bars represent respectively means ± SEM of quantitation (fold change of control) of Apc and Ccnd1 mRNA corrected for Gapdh mRNA as an internal standard. Means ± SEM with an asterisk differ (p < 0.05). (D) The bands are representative immunocomplexes performed in duplicate for c-JUN, COX-2, histone deacetylase-1 (HDAC-1), acetylated histone 3 (ACH3), and internal control β-ACTIN in colonic mucosa of mice fed a control or n-6HFD.