Table 2.
The primers for RT-PCR and CpG methylation mouse studies.
| Target | Primer Sequence a |
|---|---|
| mRNA: | |
| Fxr | F: TTAGTCTTCACCACAGCCACC |
| R: ACCTGTATACATACATTCAGCCAAC | |
| Apc | F: CTGAGCCTGGATGAGCCATT |
| R: GTGAGTCCAAGGCGAACGTC | |
| Pparγ1 | F: GTGAGACCAACAGCCTGACG |
| R: ACAGACTCGGCACTCAATGG | |
| Cox-2 | F: GAAGTCTTTGGTCTGGTGCCT |
| R: GCTCCTGCTTGAGTATGTCG | |
| Gapdh | F: CACTTGAAGGGTGGAGCCAA |
| R: AGTGATGGCATGGACTGTGG | |
| Ibabp | F: CAGGAGACGTGATTGAAAGGG |
| R: GCCCCCAGAGTAAGACTGGG | |
| Shp | F: GTACCTGAAGGGCACGATCC |
| R: AGCCTCCTGTTGCAGGTGT | |
| Ccnd1 | F: CTAAACAAGCACCCCCTCCA |
| R: GGTAACAGGGCTGTAGGCAC | |
| Methylation-specific: | |
| Fxr | F: CGTTTAGCGATGGGGTTAATTAG |
| R: CGTCTTCTTTACTTATCTAAACCTCCTT | |
| Apc | F: GAGTGTGGTTGTCGGAAATTC |
| R: CAAAAAAACGTACATAAAAAACGCT | |
| Ptsg-2 | F: TTTTAGTTAGGATTTTAGATTTCGG |
| R: ATAATACCAAAAAAACTACACCGC | |
| β-Actin | F: AATAGTTATTTTAAGTATTTATGAAATAAG |
| R: TAACTACCTCAACACCTCAAC |
a F = forward; R = reverse. Apc = adenomatous polyposis Coli; Cox-2 = cyclooxygenase-2; Ccnd1 = cyclin D1; Fxr = farsenoid-X-receptor; Gadph = glyceraldehyde dehydrogenase phosphate; Ibabp = ileal bile acid-binding protein; Pparγ1 = peroxisome proliferator-activated receptorγ1; Ptsg-2 = prostaglandin-endoperoxide synthase-2; Shp = small heterodimer protein.