Figure 5.
Aerosol transmission of JEV in mice. The mice were infected i.n. with 106.0 pfu/50 μL of the SCYA201201 or SA14-14-2 virus, respectively, and were housed in a cage with a double-walled wire partition that permitted airflow but prevented direct contact. The sentinel animals were added at 24 h post-infection. The mice were sacrificed, and their lungs were harvested at 3, 5, and 7 dpi. The SCYA201201 was marked with hollow symbols (infected with ○ and sentinel with ◇) and the SA14-14-2 was marked with solid symbols (infected with ● and sentinel with ◆). (A) The viral RNA load in the lungs was measured using real-time RT-PCR assay. The horizontal dotted lines indicate the limit of detection (**** P ˂ 0.0001). (B) The virus titer in the lung samples were determined by the plaque assay.