Figure 5.

Effect of TBI on osteoclast differentiation and colony forming unit (CFU) on bone marrow cells. (a) Mouse primary bone marrow cells were cultured with RANKL (100 ng/mL) and M-CSF (30 ng/mL) for 4 days followed by TRAP staining. After TRAP staining, TRAP + multinuclear cells (TRAP + MNCs) with more than three nuclei were scored as osteoclasts. (b) Colony forming assay was performed, stained with crystal violet, and colonies were counted * p < 0.05 and ** p < 0.01 compared with vehicle-treated control.