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. 2019 Jan 16;11(1):101. doi: 10.3390/cancers11010101

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Concomitant activation of STAT3 and SP1 is associated with poor prognosis in breast cancer. (A) SP1 binding to the RHOU promoter measured by ChIP assay in MDA-MB-231 cells cultured in the presence or absence of Mit. A. Data are mean ± SEM of the fold enrichment obtained with anti-SP1 antibodies versus IgGs in three independent experiments. ** p < 0.005 (B) Bar plot showing the gene sets significantly enriched for STAT3 and SP1 bound genes. (C) IGV genome browser pictures showing binding of both SP1 and STAT3 in the indicated cell lines. Green: STAT3; Red: SP1. Two representative genes of the two top-ranking gene sets in (B), WNT5B and JUN, are shown. (D) Kaplan-Meier plot of overall survival for breast tumor patients, as a function of time in days. Patient samples from the METABRIC database (https://www.synapse.org/#!Synapse:syn1688369/) were subdivided according to low or high SP1-S3 score (median value). (E) Results of generalized linear models correlating grade, ER status, treatment, age at diagnosis and lymph node positivity to the SP1-STAT3 score. Coefficients (estimate) of the predictors and significance are indicated.

Concomitant activation of STAT3 and SP1 is associated with poor prognosis in breast cancer. (A) SP1 binding to the RHOU promoter measured by ChIP assay in MDA-MB-231 cells cultured in the presence or absence of Mit. A. Data are mean ± SEM of the fold enrichment obtained with anti-SP1 antibodies versus IgGs in three independent experiments. ** p < 0.005 (B) Bar plot showing the gene sets significantly enriched for STAT3 and SP1 bound genes. (C) IGV genome browser pictures showing binding of both SP1 and STAT3 in the indicated cell lines. Green: STAT3; Red: SP1. Two representative genes of the two top-ranking gene sets in (B), WNT5B and JUN, are shown. (D) Kaplan-Meier plot of overall survival for breast tumor patients, as a function of time in days. Patient samples from the METABRIC database (https://www.synapse.org/#!Synapse:syn1688369/) were subdivided according to low or high SP1-S3 score (median value). (E) Results of generalized linear models correlating grade, ER status, treatment, age at diagnosis and lymph node positivity to the SP1-STAT3 score. Coefficients (estimate) of the predictors and significance are indicated.