Table 1.
Characteristics of the animals selected to investigate the oxidation of the proteins present in the uterine lavage fluids by SDS-PAGE and Western blot analysis to detect carbonylated proteins and dityrosine formation.
| Clinical Classification | Animal Identification | Neutrophil (%) | AOPP (ng/mg Protein) | Carbonyl Group (ng/mg Protein) | ||||
|---|---|---|---|---|---|---|---|---|
| CB | LNH | HNH | LNH | HNH | LNH | HNH | ||
| H | 13 | 0.1 | 0.2 | 1.7 | 3.7 | 8.2 | 0.3 | 0.7 |
| 15 | 0.3 | 1.0 | 4.5 | 12.9 | 24.5 | nd | 0.3 | |
| SCE | 3 | 29.0 | 17.7 | 19.5 | 11.3 | 16.5 | 5.8 | 7.8 |
| 17 | 5.5 | 2.3 | 48.1 | 8.6 | 32.4 | 4.8 | 34.2 | |
| EM1 | 19 | 18.0 | 65.0 | 62.2 | 16.4 | 22.1 | 2.5 | 3.2 |
| 29 | 63.9 | 78.9 | 88.5 | 3.0 | 3.0 | 0.3 | 1.7 | |
| EM2 | 30 | 39.4 | 49.9 | 52.1 | 9.8 | 11.9 | 5.7 | 6.6 |
| 37 | 5.1 | 17.4 | 24.8 | 2.4 | 4.0 | nd | 0.8 | |
H: Healthy; SCE: Subclinical Endometritis; EM1: Grade 1 Endometritis; EM2: Grade 2 Endometritis; CB: cytobrush; HNH: High Neutrophil Horn; LNH: Low Neutrophil Horn; nd: not detected.