Table 1.
High-molecular-mass components in the venom and venom sac extract detected by LC-ESI-MS.
| Peptide/Protein | Accession Number | Coverage (%) a | |||
|---|---|---|---|---|---|
| TR b−, DTT b− |
TR−, DTT+ |
TR+, DTT− |
TR+, DTT+ |
||
| acetylcholine esterase | FX985608 | 1 | |||
| apolipophorin 1 | FX985561 | 2 | 4 | 8 | 7 |
| apolipophorin 2 | FX985562 | 10 | 61 | 29 | |
| calcium-independent phospholipase A2 gamma | FX985510 | 12 | 7 | 7 | |
| carboxypeptidase D | FX985540 | 5 | 4 | 4 | |
| carboxypeptidase Q | FX985538 | 67 | 26 | ||
| CDV3 homolog | FX986049 | 8 | |||
| chymotrypsin inhibitor 1 | FX986026 | 11 | |||
| cytochrome P450 1 | FX985568 | 10 | 5 | 4 | |
| cytochrome P450 3 | FX985570 | 11 | |||
| cytochrome P450 4 | FX985571 | 4 | |||
| cytochrome P450 5 | FX985572 | 9 | 6 | 9 | |
| cytochrome P450 6 | FX985573 | 5 | 2 | ||
| cytosolic carboxypeptidase-like protein 5 | FX985541 | 4 | 7 | ||
| dishevelled homolog 3 | FX986050 | 3 | 4 | ||
| hyaluronidase | FX985505 | 5 | 68 | 47 | |
| lysosomal ProX carboxypeptidase | FX985539 | 3 | 7 | ||
| matrix metalloproteinase 14 | FX985528 | 20 | 18 | ||
| NADPH cytochrome P450 reductase | FX985574 | 5 | 6 | 5 | |
| neuroblastoma suppressor of tumorigenicity 1 | FX986056 | 7 | |||
| neuropeptide-like 1 | FX986015 | 7 | 7 | ||
| peptidyl-prolyl cis-trans isomerase 1 | FX986028 | 22 | 17 | ||
| peptidyl-prolyl cis-trans isomerase 2 | FX986029 | 4 | |||
| peptidyl-prolyl cis-trans isomerase 3 | FX986030 | 3 | 8 | ||
| peptidyl-prolyl cis-trans isomerase 4 | FX986031 | 7 | 8 | ||
| peptidyl-prolyl cis-trans isomerase 5 | FX986032 | 6 | 7 | ||
| peptidyl-prolyl cis-trans isomerase 7 | FX986034 | 1 | |||
| peptidyl-prolyl cis-trans isomerase 8 | FX986035 | 38 | |||
| pheromone binding protein 1 | FX985594 | 14 | |||
| pheromone binding protein 2 | FX985595 | 11 | |||
| pheromone binding protein 3 | FX985596 | 20 | |||
| pheromone binding protein 4 | FX985597 | 16 | |||
| pheromone binding protein 6 | FX985599 | 4 | |||
| phospholipase A2 isozyme 2 | FX985507 | 11 | 36 | 40 | |
| protein disulfide isomerase 1 | FX985548 | 8 | 14 | 8 | |
| protein disulfide isomerase 2 | FX985549 | 19 | 8 | ||
| royal jelly protein | FX986021 | 9 | |||
| UDP glucuronosyltransferase 2C1 | FX986048 | 14 | 4 | ||
| uncharacterized protein 1 | FX985636 | 4 | 5 | 3 | 6 |
| uncharacterized protein 3 | FX986042 | 19 | 64 | ||
| uncharacterized protein 4 | FX986043 | 54 | 64 | ||
| UPF0518 protein | FX986054 | 4 | 3 | 3 | 2 |
| VEGF C-like protein | FX985521 | 6 | |||
| venom allergen 1 | FX985511 | 7 | |||
| venom allergen 2 | FX985512 | 5 | |||
| venom allergen 3 | FX985513 | 21 | 5 | ||
| venom dipeptidyl peptidase 1 | FX985542 | 4 | 12 | ||
| venom dipeptidyl peptidase 2 | FX985543 | 4 | 4 | ||
| venom dipeptidyl peptidase 3 | FX985544 | 10 | 3 | 5 | |
| venom serine carboxypeptidase | FX985537 | 3 | |||
| venom serine protease 2 | FX985523 | 5 | |||
| venom serine protease 3 | FX985524 | 6 | |||
| very high density protein 1 | FX985566 | 3 | 3 | 3 | 4 |
| very high density protein 2 | FX985567 | 3 | 5 | ||
| vitellogenin 1 | FX985563 | 3 | 5 | 5 | 2 |
| vitellogenin 2 | FX985564 | 10 | |||
| waprin 1 | FX985515 | 22 | |||
| waprin 2 | FX985516 | 7 | 11 | ||
| 40S ribosomal protein SA * | FX985616 | 8 | 8 | ||
| 60S acidic ribosomal protein P0 * | FX985621 | 22 | 6 | 3 | |
| 60S ribosomal protein L10 * | FX985634 | 11 | |||
| 60S ribosomal protein L3 * | FX985633 | 10 | 7 | ||
| 60S ribosomal protein L34 * | FX985627 | 16 | |||
| 60S ribosomal protein L36 * | FX985629 | 15 | |||
| 60S ribosomal protein L4 * | FX985618 | 5 | 8 | ||
| 60S ribosomal protein L6 * | FX985625 | 5 | 11 | ||
| 60S ribosomal protein L7 * | FX985622 | 12 | |||
| 60S ribosomal protein L7a * | FX985617 | 13 | |||
| 60S ribosomal protein L9 * | FX985635 | 10 | |||
| actin, muscle * | FX985587 | 13 | 7 | 7 | |
| ATPase WRNIP1 * | FX986047 | 13 | |||
| elongation factor 1-alpha * | FX985554 | 10 | 6 | ||
| elongation factor 1-beta * | FX985557 | 5 | |||
| elongation factor 1-delta * | FX985559 | 8 | 29 | ||
| elongation factor 1-gamma * | FX985555 | 20 | 8 | 2 | |
| elongation factor 2 * | FX985553 | 4 | 4 | 2 | 8 |
| elongation factor G, mitochondrial * | FX985558 | 2 | 11 | ||
| elongation factor Tu, mitochondrial * | FX985556 | 10 | 3 | ||
| histone H2A * | FX985611 | 21 | |||
| histone H3 * | FX985610 | 15 | |||
| laminin subunit alpha 1 * | FX985614 | 4 | 6 | 5 | 8 |
| laminin subunit beta 1 * | FX985613 | 3 | 3 | 9 | |
| laminin subunit gamma 1 * | FX985612 | 3 | 3 | ||
| myosin heavy chain, muscle * | FX985575 | 4 | 3 | 11 | 8 |
| myosin heavy chain, nonmuscle * | FX985578 | 2 | 1 | 4 | |
| myosin IB * | FX985586 | 11 | 13 | ||
| myosin Ie * | FX985583 | 5 | 7 | 5 | 9 |
| myosin regulatory light chain * | FX985576 | 5 | |||
| myosin Va * | FX985582 | 2 | 5 | 4 | 8 |
| myosin VIIa * | FX985580 | 5 | 6 | 5 | |
| myosin XV * | FX985581 | 5 | 5 | 3 | 6 |
| myosin XVIIIa * | FX985584 | 2 | 5 | 4 | 3 |
| resistance to inhibitors of cholinesterase 3 * | FX986036 | 5 | |||
| transcription factor A, mitochondrial * | FX985588 | 12 | |||
| transmembrane protein 214A * | FX986055 | 10 | 4 | 4 | |
| TRPA channel d, * | FX985591 | 5 | |||
| TRPM channel d, * | FX985592 | 10 | 2 | 5 | 5 |
| TRPV channel d, * | FX985593 | 3 | |||
| voltage-gated potassium channel Shaker * | FX985601 | 9 | |||
| voltage-gated sodium channel beta subunit TipE * | FX985590 | 9 | 4 | 2 | |
| voltage-gated sodium channel Para * | FX985589 | 2 | 10 | ||
a The coverages were calculated by combining the peptide fragments with the same amino acid sequences and the same molecular masses by Peaks 8.5; b TR and DTT indicate MS/MS data with or without trypsin digestion and DTT treatment, respectively; c VEGF: Vascular Endothelial Growth Factor; d TRPA, TRPM, and TRPV channels are members of the transient receptor potential (TRP) channel superfamily; * The proteins might be derived from the venom sac.