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. 2018 Dec 30;11(1):20. doi: 10.3390/v11010020

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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7-KC reduced ZIKV budding efficiency and infectious virion production. (A) ZIKV infection was inhibited by adding 7-KC at the indicated time points following infection. Twenty-four hours post infection (hpi), total cellular RNA was harvested and ZIKV genomes were quantified using q-RT-PCR. 7-KC did not significantly alter the level of ZIKV genomes in the cells. (B) ZIKV genome copies present in the supernatants were also quantified. To determine the budding/release efficiency we compared the number of genomes present in the supernatant to cell associated genomes and determined the budding efficiency based on DMSO control. (C) ZIKV produced in the presence of 7-KC is less infectious than virus grown in DMSO control cells. The specific infectivity of the virions was determined by comparing the titer to the number of genome copies found in the supernatant (×1000). Each RNA sample was run in triplicate during each trial for a total of 3 trials. *, p < 0.05; **, p < 0.01.