Table 3.
Phage Used to Generate BIM | Bacterial Lawn | BIM Confirmation 1 | Test for Complementation | |||||
---|---|---|---|---|---|---|---|---|
Sa83 | Sa87 | J-Sa36 | Sa76 | Sa81 | J-Sa37 | |||
Sa87 | parental | S | S | S | S | S | S | S |
BIM 1 | I | S | - | S | S | S | R | |
BIM 2 | I | S | - | S | S | S | R | |
BIM 3 | NG 2 | - | - | - | - | - | - | |
BIM 4 | I | S | - | S | S | S | R | |
BIM 5 | I | S | - | I | S | S | R | |
BIM 6 | I | S | - | S | S | S | R | |
BIM 7 | I | S | - | S | S | S | R | |
BIM 8 | I | S | - | I | S | S | R | |
BIM 9 | I | S | - | I | S | S | R | |
BIM 10 | I | S | - | I | S | S | R | |
J-Sa36 | Parental | S | S | S | S | - | - | S |
BIM 1 | I | S | I | - | - | - | R | |
BIM 2 | I | I | I | - | - | - | R | |
BIM 3 | I | I | S | - | - | - | S | |
BIM 4 | I | I | I | - | - | - | S |
1 Bacteriophage-insensitive mutant (BIM) confirmation conducted using same phage as in column 1. R (red): resistant (no phage activity seen within bacterial spot); I (yellow): intermediate (phage activity seen within bacterial spot); S (green): sensitive (<10 colonies within bacterial spot), -: not tested. 2 NG: no growth; BIM was not recovered during single colony purification and is therefore presumed to be sensitive; no other testing possible.