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. 2018 Dec 20;11(1):7. doi: 10.3390/nu11010007

Figure 1.

Figure 1

Breeding scheme and spontaneous locomotor activity. (A) Experimental design illustrating the breeding scheme. Female mice (F0) were fed either a (high-fat diet) HFD or chow diet for a total of nine weeks (three weeks prior to conception, three weeks during gestation, and three weeks during lactation) to obtain the F1 HFD and F1 control (CTR) offspring, respectively. F1-HFD and CTR males were mated with naïve females to generate the F2 offspring, respectively. The F2-HFD and CTR males were mated with naïve females to obtain the F3 offspring. All offspring from both groups were maintained on an ad libitum chow diet from weaning. Colors of the mice are matched with the color codes used for the groups in subsequent graphs. (B,C) General locomotor activity in the open field. The line plot shows the distance travelled (in cm) in successive 10-min bins for male and female offspring in the F2 (B) and F3 generation (C). All values are means ± SEM. N (F2 CTR) = (10 M, 10 F), N (F2 HFD) = (10 M, 10 F), N (F3 CTR) = (8 M, 8 F), N (F3 HFD) = (8 M, 8 F), HFD = high-fat diet, CTR = control diet, F = female, M = male.