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. 2019 Jan 14;11(1):41. doi: 10.3390/toxins11010041

Figure 5.

Figure 5

Sclerotia production analysis in the WT (wild-type), ∆AfsakA and ∆AfsakA::AfsakA strains. (A) presents the phenotype of WT, ∆AfsakA and ∆AfsakA::AfsakA to sclerotia formation. All the strains were grown in the dark for 7 days at 37 °C on WKM (wickerham) media with and without the indicated osmotic stress supplements. (B) reports the number of sclerotia produced from the four replicates. The abbreviation ND stands for “no detection” of sclerotia. (C) depictures the expression levels of nsdC and nsdD genes related with sclerotial development at 48 h without stress. The 2−ΔΔCT process was used to calculate the expression levels of target gene which were normalized to β-actin as an endogenous gene. The asterisks show significant difference level when other strains were compared with WT (** p ≤ 0.01 and *** p ≤ 0.001) and the line bar in every column indicates standard errors of the four repeats.