Figure 6.

Aflatoxin B₁ (AFB₁) production analysis of WT, ∆AfsakA and ∆AfsakA::AfsakA strains. (A) AFB₁ tested by tin layer chromatograph in all the strains after being grown in dark at 29 °C for 5 days on YES agar medium with or without osmotic stress supplements. The acronym Std correspond to the AFB₁ standard. (B) the computation of AFB₁ produced from TLC (Thin Layer Chromatograph) analysis in Figure 6A. (C) the qRT-PCR results of the expression level of aflR, aflS, aflQ and aflO genes related to the aflatoxin biosynthesis at 48 h in the absence of stress agents. The line bar in every column in (B,C) indicates the standard errors of the three replicates and the asterisks show significant difference level between WT and other strains (* p ≤ 0.05, ** p ≤ 0.01 and *** p ≤ 0.001).