Figure 7.

Effect of AfsakA deletion on fungal pathogenicity on peanut seeds and maize kernel. (A) Maize kernels were treated with ∆AfsakA::AfsakA, ∆AfsakA, WT strains and water (mock) and grown for 6 days at 29 °C in the dark. (B) Peanut seeds were treated with ∆AfsakA::AfsakA, ∆AfsakA, WT strains and water (mock) and grown in the dark for 6 days at 29 °C. (C) Quantitative evaluation of the amount of conidia produced on the infected crop seeds in every strain from (A,B). The abbreviation ND stands for “no detection.” (D) TLC analysis of Aflatoxin B₁ (AFB₁) produced by both the infected maize kernels and peanut seeds. (E) Relative amount of AFB₁ produced in (D). The abbreviation Std corresponds to the AFB₁ standard. The line bar in every column indicates standard errors of three replicates and the asterisks show significant difference level between WT and other strains (** p ≤ 0.01, *** p ≤ 0.001).