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. 2019 Jan 14;11(1):41. doi: 10.3390/toxins11010041

Figure 8.

Figure 8

Subcellular allocation of AfSakA-mCherry fusion. (A) Gene replacement approach was used during construction of AfsakA(p)-AfsakA-mCherry strain. The UTR and AfsakA(p) abbreviations stand for “untranslated region” and “native promoter of AfsakA,” respectively. (B) Laser confocal scanning images of AfSakA-mCherry in vegetative mycelium. The AfSakA-mCherry strain was cultured for 16 h at 30 °C in YES liquid medium and cultured for 15, 30 min in the presence of 1.2 mol/L D-sorbitol. On the scale, 1 bar = 10 µm.