Table 3.
Primers used for qRT-PCR.
| Primers | Sequence (5′-3′) | Application |
|---|---|---|
| Q-AfsakA-F | CCCACTACCAAAGGCACTC | AfsakA detection |
| Q-AfsakA-R | GGCATCATTGAACGACCAG | |
| AflO-F | GATTGGGATGTGGTCATGCGATT | AflO qRT-PCR |
| AflO-R | GCCTGGGTCCGAAGAATGC | |
| AflQ-F | GTCGCATATGCCCCGGTCGG | AflQ qRT-PCR |
| AflQ-R | GGCAACCAGTCGGGTTCCGG | |
| AflR-F | AAAGCACCCTGTCTTCCCTAAC | AflR qRT-PCR |
| AflR-R | GAAGAGGTGGGTCAGTGTTTGTAG | |
| AflS-F | GCTCAGACTGACCGCCGCTC | AflR qRT-PCR |
| AflS-R | GCTCAGACTGACCGCCGCTC | |
| NsdC-F | GCCAGACTTGCCAATCAC | NsdC qRT-PCR |
| NsdC-R | CATCCACCTTGCCCTTTA | |
| NsdD-F | GGACTTGCGGGTCGTGCTA | NsdD qRT-PCR |
| NsdD-R | AGAACGCTGGGTCTGGTGC | |
| abaA-F | TCTTCGGTTGATGGATGATTTC | abaA qRT-PCR |
| abaA-R | CCGTTGGGAGGCTGGGT | |
| brlA-F | GCCTCCAGCGTCAACCTTC | brlA qRT-PCR |
| brlA-R | TCTCTTCAAATGCTCTTGCCTC | |
| HSP-F | CCGGCATACTATGTCTCGTCT | HSP qRT-PCR |
| HSP-R | TAGGGCCTTCGTCGAACA | |
| GPD-F | TGTCTCGGTGGTGTCCCTAT | GPD qRT-PCR |
| GPD-R | ACCATGGCTGATGGAAGACT | |
| GRE-F | GCGTATCGTCGTTACCTCATC | GRE qRT-PCR |
| GRE-R | CCTTCTCCTTTACCTCCTCGAT | |
| STL-F | CGTTTACCACGACCAGAGC | STL qRT-PCR |
| STL-R | AAGCTCAAGCCATGTGCAG | |
| Actin-F | ACGGTGTCGTCACAAACTGG | Actin qRT-PCR |
| Actin-R | CGGTTGGACTTAGGGTTGATAG |