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. 2018 Dec 22;8(1):8. doi: 10.3390/cells8010008

Figure 3.

Figure 3

Analysis of necroptosis versus apoptosis in MCF-7 cells. (A) Confirmation of necroptosis versus apoptosis in MCF-7 cells treated with SAHA (7.5 μM), letrozole (100 nM), and RG7388 (2.0 μM) individually and in combinations (SAHA + letrozole and SAHA + RG7388) using the SYTOX green staining method. (B) Analysis of necroptosis and apoptosis in MCF-7 cells with SAHA (7.5 μM), letrozole (100 nM), and RG7388 (2.0 μM) individually and in combinations (SAHA + letrozole and SAHA + RG7388) for 24 h, with SYTOX green and DEVD-amc using a spectrofluorometric assay in a 96-well plate. Data are presented in the bar graph are mean ± SD, from minimum of three independent experiments. The level of significance is indicated as *** p < 0.001 compared to control.