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Phenotypic analysis in spike-in samples. The HCT116–leucocyte suspension at 1:1,000,000 dilution was prepared into a monolayer with a self-assembled cell array (SACA) chip and was stained with anti-epithelial cell adhesion molecule (EpCAM) conjugated with fluorescein isothiocyanate (FITC), anti-cluster of differentiation 45 (CD45)-AF594, and Hoechst 33258. The target cell is marked with a white arrow.
